Recombinant Subunit Vaccine Candidate against the Bovine Viral Diarrhea Virus

Vista sencilla de ítem
dc.contributor.affiliationAvello, V., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.affiliationSalazar, S., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.affiliationGonzález, E.E., Department of Medicine, Division of Gastroenterology, Miller School of Medicine, University of Miami, Miami, FL 33146, United States
dc.contributor.affiliationCampos, P., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.affiliationManríque, V., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.affiliationMathieu, C., Virology Section of the SAG’s Sub-Department Network of Animal Health Laboratories, Lo Aguirre, Santiago de Chile, 9020000, Chile
dc.contributor.affiliationHugues, F., Pathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Vicente Méndez 595P.O. Box 537, Chillán, Chile
dc.contributor.affiliationCabezas, I., Pathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Vicente Méndez 595P.O. Box 537, Chillán, Chile
dc.contributor.affiliationGädicke, P., Pathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Vicente Méndez 595P.O. Box 537, Chillán, Chile
dc.contributor.affiliationParra, N.C., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.affiliationAcosta, J., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.affiliationSánchez, O., Pharmacology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290P.O. Box 160C, Concepción, Chile
dc.contributor.affiliationGonzález, A., Faculty of Basic Sciences, University of Medellin, Cra. 87 No 30-65, Medellin, 050026, Colombia
dc.contributor.affiliationMontesino, R., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile
dc.contributor.authorAvello V
dc.contributor.authorSalazar S
dc.contributor.authorGonzález E.E
dc.contributor.authorCampos P
dc.contributor.authorManríque V
dc.contributor.authorMathieu C
dc.contributor.authorHugues F
dc.contributor.authorCabezas I
dc.contributor.authorGädicke P
dc.contributor.authorParra N.C
dc.contributor.authorAcosta J
dc.contributor.authorSánchez O
dc.contributor.authorGonzález A
dc.contributor.authorMontesino R.
dc.date.accessioned2024-12-27T20:52:21Z
dc.date.available2024-12-27T20:52:21Z
dc.date.issued2024
dc.descriptionMultivalent live-attenuated or inactivated vaccines are often used to control the bovine viral diarrhea disease (BVD). Still, they retain inherent disadvantages and do not provide the expected protection. This study developed a new vaccine prototype, including the external segment of the E2 viral protein from five different subgenotypes selected after a massive screening. The E2 proteins of every subgenotype (1aE2, 1bE2, 1cE2, 1dE2, and 1eE2) were produced in mammalian cells and purified by IMAC. An equimolar mixture of E2 proteins formulated in an oil-in-water adjuvant made up the vaccine candidate, inducing a high humoral response at 50, 100, and 150 µg doses in sheep. A similar immune response was observed in bovines at 50 µg. The cellular response showed a significant increase in the transcript levels of relevant Th1 cytokines, while those corresponding to the Th2 cytokine IL-4 and the negative control were similar. High levels of neutralizing antibodies against the subgenotype BVDV1a demonstrated the effectiveness of our vaccine candidate, similar to that observed in the sera of animals vaccinated with the commercial vaccine. These results suggest that our vaccine prototype could become an effective recombinant vaccine against the BVD. © 2024 by the authors.
dc.identifier.doi10.3390/ijms25168734
dc.identifier.instnameinstname:Universidad de Medellínspa
dc.identifier.issn16616596
dc.identifier.reponamereponame:Repositorio Institucional Universidad de Medellínspa
dc.identifier.repourlrepourl:https://repository.udem.edu.co/
dc.identifier.urihttp://hdl.handle.net/11407/8753
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)spa
dc.publisher.facultyFacultad de Ciencias Básicasspa
dc.relation.citationissue16
dc.relation.citationvolume25
dc.relation.isversionofhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85202677301&doi=10.3390%2fijms25168734&partnerID=40&md5=cf944566ba002d33fa9b4805b85c3f95
dc.relation.referencesGoyal, S.M., Ridpath, J.F., (2005) Bovine Viral Diarrhea Virus: Diagnosis, Management and Control, , Blackwell Publishing Professional, Ames, IA, USA
dc.relation.referencesGiammarioli, M., Ceglie, L., Rossi, E., Bazzucchi, M., Casciari, C., Petrini, S., De Mia, G.M., Increased Genetic Diversity of Bvdv-1: Recent Findings and Implications Thereof (2015) Virus Genes, 50, pp. 147-151. , 25349062
dc.relation.referencesYesilbag, K., Alpay, G., Becher, P., Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus (2017) Viruses, 9. , 28587150
dc.relation.referencesDeng, M., Chen, N., Guidarini, C., Xu, Z., Zhang, J., Cai, L., Yuan, S., Metcalfe, L., Prevalence and Genetic Diversity of Bovine Viral Diarrhea Virus in Dairy Herds of China (2020) Vet. Microbiol, 242. , 32122580
dc.relation.referencesSantman-Berends, I., Mars, M.H., Van Duijn, L., Van den Broek, K.W.H., Van Schaik, G., A Quantitative Risk-Analysis for Introduction of Bovine Viral Diarrhoea Virus in the Netherlands through Cattle Imports (2017) Prev. Vet. Med, 146, pp. 103-113. , 28992914
dc.relation.referencesKelling, C.L., Evolution of Bovine Viral Diarrhea Virus Vaccines (2004) Vet. Clin. N. Am. Food Anim. Pract, 20, pp. 115-129. , 15062478
dc.relation.referencesBolin, S.R., Ridpath, J.F., Specificity of Neutralizing and Precipitating Antibodies Induced in Healthy Calves by Monovalent Modified-Live Bovine Viral Diarrhea Virus Vaccines (1989) Am. J. Vet. Res, 50, pp. 817-821. , 2548419
dc.relation.referencesCortese, V.S., Whittaker, R., Ellis, J., Ridpath, J.F., Bolin, S.R., Specificity and Duration of Neutralizing Antibodies Induced in Healthy Cattle after Administration of a Modified-Live Virus Vaccine against Bovine Viral Diarrhea (1998) Am. J. Vet. Res, 59, pp. 848-850. , 9659549
dc.relation.referencesFulton, R.W., Burge, L.J., Bovine Viral Diarrhea Virus Types 1 and 2 Antibody Response in Calves Receiving Modified Live Virus or Inactivated Vaccines (2000) Vaccine, 19, pp. 264-274
dc.relation.referencesMcClurkin, A.W., Littledike, E.T., Cutlip, R.C., Frank, G.H., Coria, M.F., Bolin, S.R., Production of Cattle Immunotolerant to Bovine Viral Diarrhea Virus (1984) Can. J. Comp. Med, 48, pp. 156-161. , 6326980
dc.relation.referencesRidpath, J.F., Immunology of Bvdv Vaccines (2013) Biologicals, 41, pp. 14-19
dc.relation.referencesFalkenberg, S.M., Dassanayake, R.P., Crawford, L., Sarlo Davila, K., Boggiatto, P., Response to Bovine Viral Diarrhea Virus in Heifers Vaccinated with a Combination of Multivalent Modified Live and Inactivated Viral Vaccines (2023) Viruses, 15. , 36992412
dc.relation.referencesNewcomer, B.W., Walz, P.H., Givens, M.D., Wilson, A.E., Efficacy of Bovine Viral Diarrhea Virus Vaccination to Prevent Reproductive Disease: A Meta-Analysis (2015) Theriogenology, 83, pp. 360-365.e1
dc.relation.referencesPatel, J.R., Shilleto, R.W., Williams, J., Alexander, D.C., Prevention of Transplacental Infection of Bovine Foetus by Bovine Viral Diarrhoea Virus through Vaccination (2002) Arch. Virol, 147, pp. 2453-2463. , 12491111
dc.relation.referencesAntos, A., Miroslaw, P., Rola, J., Polak, M.P., Vaccination Failure in Eradication and Control Programs for Bovine Viral Diarrhea Infection (2021) Front. Vet. Sci, 8. , 34268349
dc.relation.referencesNewcomer, B.W., Givens, D., Diagnosis and Control of Viral Diseases of Reproductive Importance: Infectious Bovine Rhinotracheitis and Bovine Viral Diarrhea (2016) Vet. Clin. N. Am. Food Anim. Pract, 32, pp. 425-441
dc.relation.referencesBrownlie, J., Clarke, M.C., Hooper, L.B., Bell, G.D., Protection of the Bovine Fetus from Bovine Viral Diarrhoea Virus by Means of a New Inactivated Vaccine (1995) Vet. Rec, 137, pp. 58-62. , 8533232
dc.relation.referencesAl-Kubati, A.A.G., Hussen, J., Kandeel, M., Al-Mubarak, A.I.A., Hemida, M.G., Recent Advances on the Bovine Viral Diarrhea Virus Molecular Pathogenesis, Immune Response, and Vaccines Development (2021) Front. Vet. Sci, 8
dc.relation.referencesLaureyns, J., Ribbens, S., de Kruif, A., Control of Bovine Virus Diarrhoea at the Herd Level: Reducing the Risk of False Negatives in the Detection of Persistently Infected Cattle (2010) Vet. J, 184, pp. 21-26
dc.relation.referencesAitkenhead, H., Riedel, C., Cowieson, N., Rumenapf, H.T., Stuart, D.I., El Omari, K., Structural Comparison of Typical and Atypical E2 Pestivirus Glycoproteins (2024) Structure, 32, p. 273
dc.relation.referencesDonofrio, G., Bottarelli, E., Sandro, C., Flammini, C.F., Expression of Bovine Viral Diarrhea Virus Glycoprotein E2 as a Soluble Secreted form in a Mammalian Cell Line (2006) Clin. Vaccine Immunol, 13, pp. 698-701. , 16760329
dc.relation.referencesNogarol, C., Decaro, N., Bertolotti, L., Colitti, B., Iotti, B., Petrini, S., Lucente, M.S., Profiti, M., Pestivirus Infection in Cattle Dairy Farms: E2 Glycoprotein Elisa Reveals the Presence of Bovine Viral Diarrhea Virus Type 2 in Northwestern Italy (2017) BMC Vet. Res, 13. , 29202756
dc.relation.referencesCouvreur, B., Letellier, C., Olivier, F., Dehan, P., Elouahabi, A., Vandenbranden, M., Ruysschaert, J.M., Kerkhofs, P., Sequence-Optimised E2 Constructs from Bvdv-1b and Bvdv-2 for DNA Immunisation in Cattle (2007) Vet. Res, 38, pp. 819-834
dc.relation.referencesvan Drunen Littel-van den Hurk, S., Lawman, Z., Wilson, D., Luxembourg, A., Ellefsen, B., van den Hurk, J.V., Hannaman, D., Electroporation Enhances Immune Responses and Protection Induced by a Bovine Viral Diarrhea Virus DNA Vaccine in Newborn Calves with Maternal Antibodies (2010) Vaccine, 28, pp. 6445-6454. , 20670907
dc.relation.referencesEl-Attar, L.M.R., Thomas, C., Luke, J., Williams, J.A., Brownlie, J., Enhanced Neutralising Antibody Response to Bovine Viral Diarrhoea Virus (Bvdv) Induced by DNA Vaccination in Calves (2015) Vaccine, 33, pp. 4004-4012. , 26079613
dc.relation.referencesLeng, D., Yamada, S., Chiba, Y., Yoneyama, S., Sakai, Y., Hikono, H., Murakami, K., Co-Administration of a Plasmid Encoding Cd40 or Cd63 Enhances the Immune Responses to a DNA Vaccine against Bovine Viral Diarrhea Virus in Mice (2022) J. Vet. Med. Sci, 84, pp. 1175-1184. , 35793950
dc.relation.referencesChung, Y.C., Cheng, L.T., Zhang, J.Y., Wu, Y.J., Liu, S.S., Chu, C.Y., Recombinant E2 Protein Enhances Protective Efficacy of Inactivated Bovine Viral Diarrhea Virus 2 Vaccine in a Goat Model (2018) BMC Vet. Res, 14. , 29940938
dc.relation.referencesThomas, C., Young, N.J., Heaney, J., Collins, M.E., Brownlie, J., Evaluation of Efficacy of Mammalian and Baculovirus Expressed E2 Subunit Vaccine Candidates to Bovine Viral Diarrhoea Virus (2009) Vaccine, 27, pp. 2387-2393. , 19428855
dc.relation.referencesNelson, G., Marconi, P., Periolo, O., La Torre, J., Alvarez, M.A., Immunocompetent Truncated E2 Glycoprotein of Bovine Viral Diarrhea Virus (Bvdv) Expressed in Nicotiana Tabacum Plants: A Candidate Antigen for New Generation of Veterinary Vaccines (2012) Vaccine, 30, pp. 4499-4504
dc.relation.referencesPecora, A., Malacari, D.A., Perez Aguirreburualde, M.S., Bellido, D., Nunez, M.C., Dus Santos, M.J., Escribano, J.M., Wigdorovitz, A., Development of an Apc-Targeted Multivalent E2-Based Vaccine against Bovine Viral Diarrhea Virus Types 1 and 2 (2015) Vaccine, 33, pp. 5163-5171
dc.relation.referencesLo, Y.T., Ryan, M.D., Luke, G.A., Chang, W.C., Wu, H.C., Immunogenicity of a Secreted, C-Terminally Truncated, Form of Bovine Viral Diarrhea Virus E2 Glycoprotein as a Potential Candidate in Subunit Vaccine Development (2023) Sci. Rep, 13
dc.relation.referencesWang, Y., Feng, B., Niu, C., Jia, S., Sun, C., Wang, Z., Jiang, Y., Xu, Y., Dendritic Cell Targeting of Bovine Viral Diarrhea Virus E2 Protein Expressed by Lactobacillus Casei Effectively Induces Antigen-Specific Immune Responses via Oral Vaccination (2019) Viruses, 11. , 31242608
dc.relation.referencesGonzález, A., Montesino, R., Maura, R., Hugues, F., Cabezas, O., Altamirano, C., Sánchez, O., Toledo, J.R., Characterisation of a New Molecule Based on Two E2 Sequences from Bovine Viral Diarrhoea-Mucosal Disease Virus Fused to the Human Immunoglobulin Fc Fragment (2021) J. Vet. Res, 65, pp. 27-37. , 33817392
dc.relation.referencesHugues, F., Cabezas, I., Garigliany, M., Rivas, F., Casanova, T., Gonzalez, E.E., Sanchez, O., Inostroza-Michael, O., First Report of Bovine Viral Diarrhea Virus Subgenotypes 1d and 1e in Southern Chile (2023) Virol. J, 20, p. 205. , 37679808
dc.relation.referencesPizarro-Lucero, J., Celedon, M.O., Aguilera, M., de Calisto, A., Molecular Characterization of Pestiviruses Isolated from Bovines in Chile (2006) Vet. Microbiol, 115, pp. 208-217
dc.relation.referencesFernandez, F., Costantini, V., Barrandeguy, M., Parreno, V., Schiappacassi, G., Maliandi, F., Leunda, M., Odeon, A., Evaluation of Experimental Vaccines for Bovine Viral Diarrhea in Bovines, Ovines and Guinea Pigs (2009) Rev. Argent. Microbiol, 41, pp. 86-91. , 19623897
dc.relation.referencesAbdisa, T., Review on Practical Guidance of Veterinary Clinical Diagnostic Approach (2017) Int. J. Vet. Sci. Res, 3, pp. 030-049
dc.relation.referencesReece, W.O., Erickson, H.H., Goff, J.P., Uemura, E.E., (2015) Dukes’ Physiology of Domestic Animals, , 13th ed., Wiley-Blackwell, Hoboken, NJ, USA
dc.relation.referencesLivak, K.J., Schmittgen, T.D., Analysis of Relative Gene Expression Data Using Real-Time Quantitative Pcr and the 2(-Delta Delta C(T)) Method (2001) Methods, 25, pp. 402-408
dc.relation.referencesHarpin, S., Hurley, D.J., Mbikay, M., Talbot, B., Elazhary, Y., Vaccination of Cattle with a DNA Plasmid Encoding the Bovine Viral Diarrhoea Virus Major Glycoprotein E2 (1999) J. Gen. Virol, 80, pp. 3137-3144
dc.relation.referencesLiang, R., Babiuk, L.A., Hurk, S.-V., Compatibility of Plasmids Encoding Bovine Viral Diarrhea Virus Type 1 and Type 2 E2 in a Single DNA Vaccine Formulation (2007) Vaccine, 25, pp. 5994-6006
dc.relation.referencesNobiron, I., Thompson, I., Brownlie, J., Collins, M.E., Cytokine Adjuvancy of Bvdv DNA Vaccine Enhances both Humoral and Cellular Immune Responses in Mice (2001) Vaccine, 19, pp. 4226-4235
dc.relation.referencesHurk, S.-V., Lawman, Z., Snider, M., Wilson, D., Hurk, J., Ellefsen, B., Hannaman, D., Two Doses of Bovine Viral Diarrhea Virus DNA Vaccine Delivered by Electroporation Induce Long-Term Protective Immune Responses (2013) Clin. Vaccine Immunol, 20, pp. 166-173
dc.relation.referencesLiang, R., van den Hurk, J.V., Landi, A., Lawman, Z., Deregt, D., Townsend, H., Babiuk, L.A., van Drunen Littel-van den Hurk, S., DNA Prime Protein Boost Strategies Protect Cattle from Bovine Viral Diarrhea Virus Type 2 Challenge (2008) J. Gen. Virol, 89, pp. 453-466. , 18198376
dc.relation.referencesCai, D., Song, Q., Duan, C., Wang, S., Wang, J., Zhu, Y., Enhanced Immune Responses to E2 Protein and DNA Formulated with Isa 61 Vg Administered as a DNA Prime-Protein Boost Regimen against Bovine Viral Diarrhea Virus (2018) Vaccine, 36, pp. 5591-5599. , 30077483
dc.relation.referencesPerez Aguirreburualde, M.S., Gomez, M.C., Ostachuk, A., Wolman, F., Albanesi, G., Pecora, A., Odeon, A., Dus Santos, M.J., Efficacy of a Bvdv Subunit Vaccine Produced in Alfalfa Transgenic Plants (2013) Vet. Immunol. Immunopathol, 151, pp. 315-324. , 23291101
dc.relation.referencesYang, N., Zhang, J., Xu, M., Yi, J., Wang, Z., Wang, Y., Chen, C., Virus-Like Particles Vaccines Based on Glycoprotein E0 and E2 of Bovine Viral Diarrhea Virus Induce Humoral Responses (2022) Front. Microbiol, 13. , 36439839
dc.relation.referencesKatsura, M., Fukushima, M., Kameyama, K.I., Kokuho, T., Nakahira, Y., Takeuchi, K., Novel Bovine Viral Diarrhea Virus (Bvdv) Virus-Like Particle Vaccine Candidates Presenting the E2 Protein Using the Spytag/Spycatcher System Induce a Robust Neutralizing Antibody Response in Mice (2023) Arch. Virol, 168, p. 49. , 36609880
dc.relation.referencesBruschke, C.J., Moormann, R.J., van Oirschot, J.T., van Rijn, P.A., A Subunit Vaccine Based on Glycoprotein E2 of Bovine Virus Diarrhea Virus Induces Fetal Protection in Sheep against Homologous Challenge (1997) Vaccine, 15, pp. 1940-1945. , 9413105
dc.relation.referencesBruschke, C.J., van Oirschot, J.T., van Rijn, P.A., An Experimental Multivalent Bovine Virus Diarrhea Virus E2 Subunit Vaccine and Two Experimental Conventionally Inactivated Vaccines Induce Partial Fetal Protection in Sheep (1999) Vaccine, 17, pp. 1983-1991. , 10217598
dc.relation.referencesChowdhury, S.I., Pannhorst, K., Sangewar, N., Pavulraj, S., Wen, X., Stout, R.W., Mwangi, W., Paulsen, D.B., Bohv-1-Vectored Bvdv-2 Subunit Vaccine Induces Bvdv Cross-Reactive Cellular Immune Responses and Protects against Bvdv-2 Challenge (2021) Vaccines, 9
dc.relation.referencesPecora, A., Aguirreburualde, M.S., Aguirreburualde, A., Leunda, M.R., Odeon, A., Chiavenna, S., Bochoeyer, D., Dus Santos, M.J., Safety and Efficacy of an E2 Glycoprotein Subunit Vaccine Produced in Mammalian Cells to Prevent Experimental Infection with Bovine Viral Diarrhoea Virus in Cattle (2012) Vet. Res. Commun, 36, pp. 157-164
dc.relation.referencesGomez-Romero, N., Arias, C.F., Verdugo-Rodriguez, A., Lopez, S., Valenzuela-Moreno, L.F., Cedillo-Pelaez, C., Basurto-Alcantara, F.J., Immune Protection Induced by E2 Recombinant Glycoprotein of Bovine Viral Diarrhea Virus in a Murine Model (2023) Front. Vet. Sci, 10
dc.relation.referencesCheng, Y., Tu, S., Chen, T., Zou, J., Wang, S., Jiang, M., Tian, S., Zhou, H., Evaluation of the Mucosal Immunity Effect of Bovine Viral Diarrhea Virus Subunit Vaccine E2fc and E2ft (2023) Int. J. Mol. Sci, 24. , 36835584
dc.relation.referencesMosena, A.C.S., Falkenberg, S.M., Ma, H., Casas, E., Dassanayake, R.P., Walz, P.H., Canal, C.W., Neill, J.D., Multivariate Analysis as a Method to Evaluate Antigenic Relationships between Bvdv Vaccine and Field Strains (2020) Vaccine, 38, pp. 5764-5772. , 32690424
dc.relation.referencesVega, S., Rosell, R., Paton, D.J., Orden, J.A., de la Fuente, R., Antigenic Characterization of Bovine Viral Diarrhoea Virus Isolates from Spain with a Panel of Monoclonal Antibodies (2000) J. Vet. Med. B Infect. Dis. Vet. Public. Health, 47, pp. 701-706. , 11244871
dc.relation.referencesBecher, P., Avalos Ramirez, R., Orlich, M., Cedillo Rosales, S., Konig, M., Schweizer, M., Stalder, H., Thiel, H.J., Genetic and Antigenic Characterization of Novel Pestivirus Genotypes: Implications for Classification (2003) Virology, 311, pp. 96-104
dc.relation.referencesKlimowicz-Bodys, M.D., Polak, M.P., Ploneczka-Janeczko, K., Bagnicka, E., Zbroja, D., Rypula, K., Lack of Fetal Protection against Bovine Viral Diarrhea Virus in a Vaccinated Heifer (2022) Viruses, 14
dc.relation.referencesFulton, R.W., Ridpath, J.F., Confer, A.W., Saliki, J.T., Burge, L.J., Payton, M.E., Bovine Viral Diarrhoea Virus Antigenic Diversity: Impact on Disease and Vaccination Programmes (2003) Biologicals, 31, pp. 89-95
dc.relation.referencesVan Campen, H., Vorpahl, P., Huzurbazar, S., Edwards, J., Cavender, J., A case report: Evidence for Type 2 Bovine Viral Diarrhea Virus (Bvdv)-Associated Disease in Beef Herds Vaccinated with a Modified-Live Type 1 Bvdv Vaccine (2000) J. Vet. Diagn. Investig, 12, pp. 263-265. , 10826842
dc.relation.referencesDowney-Slinker, E.D., Ridpath, J.F., Sawyer, J.E., Skow, L.C., Herring, A.D., Antibody Titers to Vaccination Are Not Predictive of Level of Protection against a Bvdv Type 1b Challenge in Bos Indicus-Bos Taurus Steers (2016) Vaccine, 34, pp. 5053-5059
dc.relation.referencesBeer, M., Hehnen, H.R., Wolfmeyer, A., Poll, G., Kaaden, O.R., Wolf, G., A New Inactivated Bvdv Genotype I and II Vaccine. An Immunisation and Challenge Study with Bvdv Genotype I (2000) Vet. Microbiol, 77, pp. 195-208
dc.relation.referencesBolin, S.R., Ridpath, J.F., Assessment of Protection from Systemic Infection or Disease Afforded by Low to Intermediate Titers of Passively Acquired Neutralizing Antibody against Bovine Viral Diarrhea Virus in Calves (1995) Am. J. Vet. Res, 56, pp. 755-759. , 7653884
dc.relation.referencesRidpath, J.F., Practical significance of heterogeneity among Bvdv strains: Impact of Biotype and Genotype on U.S. Control Programs (2005) Prev. Vet. Med, 72, pp. 17-30. , 16183156
dc.relation.referencesPlatt, R., Widel, P.W., Kesl, L.D., Roth, J.A., Comparison of Humoral and Cellular Immune Responses to a Pentavalent Modified Live Virus Vaccine in Three Age Groups of Calves with Maternal Antibodies, before and after Bvdv Type 2 Challenge (2009) Vaccine, 27, pp. 4508-4519. , 19446589
dc.relation.referencesVilcek, S., Herring, A.J., Herring, J.A., Nettleton, P.F., Lowings, J.P., Paton, D.J., Pestiviruses Isolated from Pigs, Cattle and Sheep Can Be Allocated into at Least Three Genogroups Using Polymerase Chain Reaction and Restriction Endonuclease Analysis (1994) Arch. Virol, 136, pp. 309-323
dc.relation.referencesBirnboim, H.C., Doly, J., A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA (1979) Nucleic Acids Res, 7, pp. 1513-1523
dc.relation.referencesLaemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 (1970) Nature, 227, pp. 680-685
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceInternational Journal of Molecular Sciences
dc.sourceInt. J. Mol. Sci.
dc.sourceScopus
dc.subjectBovine viral diarrhea viruseng
dc.subjectBVDV subgenotypeseng
dc.subjectE2 glycoproteineng
dc.subjectSubunit vaccineeng
dc.titleRecombinant Subunit Vaccine Candidate against the Bovine Viral Diarrhea Viruseng
dc.typeArticle
dc.type.localArtículo de revistaspa
dc.type.versioninfo:eu-repo/semantics/publishedVersion

Archivos

Colecciones

Indexados Scopus