Development and characterization of a novel variant of long-acting bovine follicle-stimulating hormone (brscFSH)

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dc.contributor.affiliationCabeza, O.I., Departamento de Ciencias Clínicas, Facultad de Veterinaria, Universidad de Concepción, Chile
dc.contributor.affiliationParra, N., Departamento de Farmacología, Universidad de Concepción, Chile
dc.contributor.affiliationCerro, R., Centro de Biotecnología y Biomedicina Spa., Concepción, Chile
dc.contributor.affiliationMansilla, R., Centro de Biotecnología y Biomedicina Spa., Concepción, Chile
dc.contributor.affiliationSanchez, R.Z., Centro de Biotecnología y Biomedicina Spa., Concepción, Chile
dc.contributor.affiliationGutierrez-Reinoso, M., Departamento de Ciencia Animal, Facultad de Veterinaria, Universidad de Concepción, Chile, Facultad de Ciencias Agropecuarias y Recursos Naturales, Carrera de medicina, Universidad Técnica de Cotopaxi (UTC), Latacunga, 050150, Ecuador
dc.contributor.affiliationEscribano, E.H., Centro de Biotecnología y Biomedicina Spa., Concepción, Chile
dc.contributor.affiliationCastillo, R., Vetagro Patagonia Ltda. Coyhaique, Chile
dc.contributor.affiliationRodriguez-Alvarez, L., Departamento de Ciencia Animal, Facultad de Veterinaria, Universidad de Concepción, Chile
dc.contributor.affiliationTavares, K., Núcleo de Biologia Experimental (Nubex), Universidade de Fortaleza (Unifor), CE, Fortaleza, Brazil, FortGen Technologies, CE, Fortaleza, Brazil
dc.contributor.affiliationGaudencio, S., Núcleo de Biologia Experimental (Nubex), Universidade de Fortaleza (Unifor), CE, Fortaleza, Brazil, FortGen Technologies, CE, Fortaleza, Brazil
dc.contributor.affiliationMartins, L., Núcleo de Biologia Experimental (Nubex), Universidade de Fortaleza (Unifor), CE, Fortaleza, Brazil, FortGen Technologies, CE, Fortaleza, Brazil
dc.contributor.affiliationHugues, F.I., Departamento de Ciencias Clínicas, Facultad de Veterinaria, Universidad de Concepción, Chile
dc.contributor.affiliationAcosta, J., Departamento de Fisiopatología, Universidad de Concepción, Chile
dc.contributor.affiliationMoreno, E., Facultad de Ciencias Básicas, Universidad de Medellín, Medellín, 050026, Colombia
dc.contributor.affiliationMontesino, R., Departamento de Fisiopatología, Universidad de Concepción, Chile
dc.contributor.affiliationGarcía-Herreros, M., Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Santarém, 2005-048, Portugal
dc.contributor.affiliationCasanova, F.C., Departamento de Farmacología, Universidad de Concepción, Chile
dc.contributor.affiliationToledo, J.R., Centro de Biotecnología y Biomedicina Spa., Concepción, Chile, Departamento de Fisiopatología, Universidad de Concepción, Chile
dc.contributor.affiliationSanchez, O., Centro de Biotecnología y Biomedicina Spa., Concepción, Chile, Departamento de Farmacología, Universidad de Concepción, Chile
dc.contributor.authorCabeza O.I
dc.contributor.authorParra N
dc.contributor.authorCerro R
dc.contributor.authorMansilla R
dc.contributor.authorSanchez R.Z
dc.contributor.authorGutierrez-Reinoso M
dc.contributor.authorEscribano E.H
dc.contributor.authorCastillo R
dc.contributor.authorRodriguez-Alvarez L
dc.contributor.authorTavares K
dc.contributor.authorGaudencio S
dc.contributor.authorMartins L
dc.contributor.authorHugues F.I
dc.contributor.authorAcosta J
dc.contributor.authorMoreno E
dc.contributor.authorMontesino R
dc.contributor.authorGarcía-Herreros M
dc.contributor.authorCasanova F.C
dc.contributor.authorToledo J.R
dc.contributor.authorSanchez O.
dc.date.accessioned2024-12-27T20:52:17Z
dc.date.available2024-12-27T20:52:17Z
dc.date.issued2024
dc.descriptionAssisted reproduction is a key aspect of modern animal breeding, providing valuable assistance in improving breeding programs. In this field, the administration of exogenous hormones, such as follicle-stimulating hormone (FSH), plays a crucial role in the induction of multiple ovulations. However, commercial FSH used in veterinary practice has been derived primarily from pituitary glands, obtained mostly from pigs for nearly four decades. Although these hormones have contributed significantly to the advancement of assisted reproductive techniques, they have certain limitations that warrant further improvements. These limitations include contamination with luteinizing hormone (LH), the potential risk of pathogen contamination, the potential to trigger an immune response in non-pig species, and the short half-life in circulation, requiring the implementation of complex 8-dose superovulation schedules. Our research team has developed and characterized a new variant of bovine follicle-stimulating hormone (bscrFSH) to address these limitations. The new hormone is produced recombinantly in CHO cell cultures, with a specific productivity of about 30 pg/cell/day. The bscrFSH can be purified to a high purity of 97 % using a single step of immobilized metal affinity chromatography (IMAC). N-glycan analysis of bscrFSH showed that approximately 74 % of the glycans corresponded to charged structures, including mono-, di-, tri-, and tetra-sialylated glycans. Superovulation trials conducted in cattle revealed that bscrFSH, administered at a total dose of about 0.5 μg per kg of body weight, using a decrescent schedule of 4 doses with 24-h intervals, resulted in an average yield of 8–12 transferable embryos per animal. Further research is required; however, the preliminary findings indicate that bscrFSH, currently packaged under the provisional brand name of Cebitropin B, holds potential as a commercial product for assisted reproduction in ruminants. © 2024 Elsevier Inc.
dc.identifier.doi10.1016/j.theriogenology.2024.05.038
dc.identifier.instnameinstname:Universidad de Medellínspa
dc.identifier.issn0093691X
dc.identifier.reponamereponame:Repositorio Institucional Universidad de Medellínspa
dc.identifier.repourlrepourl:https://repository.udem.edu.co/
dc.identifier.urihttp://hdl.handle.net/11407/8737
dc.language.isoeng
dc.publisherElsevier Inc.spa
dc.publisher.facultyFacultad de Ciencias Básicasspa
dc.relation.citationendpage86
dc.relation.citationstartpage76
dc.relation.citationvolume226
dc.relation.isversionofhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85195471567&doi=10.1016%2fj.theriogenology.2024.05.038&partnerID=40&md5=59cb061fad054eb85a9a98feae6fc7b3
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dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceTheriogenology
dc.sourceTheriogenology
dc.sourceScopus
dc.subjectAssisted reproductioneng
dc.subjectFollicle stimulating hormoneeng
dc.subjectRecombinant proteineng
dc.subjectSuperovulationeng
dc.titleDevelopment and characterization of a novel variant of long-acting bovine follicle-stimulating hormone (brscFSH)eng
dc.typeArticle
dc.type.localArtículo de revistaspa
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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