Theoretical Analysis of the Hydrolysis Mechanism of Biopolymers in the Anaerobic Digestion Process of Livestock Manure

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dc.contributor.affiliationVillegas-Moncada S., Faculty of Engineering, University of Medellín, Antioquia, Medellín, 50026, Colombia
dc.contributor.affiliationLuna-delRisco M., Faculty of Engineering, University of Medellín, Antioquia, Medellín, 50026, Colombia
dc.contributor.affiliationArroyave-Quiceno C., Faculty of Engineering, University of Medellín, Antioquia, Medellín, 50026, Colombia
dc.contributor.affiliationGonzález-Palacio M., Faculty of Engineering, University of Medellín, Antioquia, Medellín, 50026, Colombia
dc.contributor.affiliationPeláez-Jaramillo C., Department of Chemistry, University of Antioquia, Antioquia, Medellín, 050010, Colombia
dc.contributor.authorVillegas-Moncada S.
dc.contributor.authorLuna-delRisco M.
dc.contributor.authorArroyave-Quiceno C.
dc.contributor.authorGonzález-Palacio M.
dc.contributor.authorPeláez-Jaramillo C.
dc.date.accessioned2025-09-08T14:23:28Z
dc.date.available2025-09-08T14:23:28Z
dc.date.issued2025
dc.descriptionOver the past two decades, modeling the hydrolysis stage has been recognized as critical for understanding its behavior and determining optimal operating conditions for anaerobic digestion (AD). Traditional approaches, such as first-order and Michaelis–Menten kinetic models, account for substrate concentration and enzymatic activity, respectively, but neglect mass-transfer effects. In this work, we propose a semi-empirical model that integrates enzymatic catalysis with molecular diffusion phenomena in the microbial boundary layer. We derive a hydrolysis rate expression by combining Michaelis–Menten kinetics with Fick’s law of diffusion and validate it against experimental data from a thermophilic batch reactor treating cattle manure (55 ∘C, 62 gVSL-1). Compared to the first-order model (R2 = 0.940), our model achieves a superior fit (R2 = 0.973), demonstrating that diffusion resistance can significantly influence hydrolysis kinetics. By formulating the kinetic model in terms of explicit biochemical and mass-transfer parameters (rh,max, KM, kd, α), it becomes possible to identify optimal operational strategies for enhancing hydrolysis efficiency. The results indicate that coupling enzymatic catalysis with diffusion provides a more accurate theoretical description than the first-order model and enables improved prediction of biopolymer solubilization in AD. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
dc.identifier.doi10.1007/s12155-025-10873-5
dc.identifier.instnameinstname:Universidad de Medellínspa
dc.identifier.issn19391234
dc.identifier.reponamereponame:Repositorio Institucional Universidad de Medellínspa
dc.identifier.repourlrepourl:https://repository.udem.edu.co/
dc.identifier.urihttps://hdl.handle.net/11407/9061
dc.language.isoeng
dc.publisher.facultyFacultad de Ingenieríasspa
dc.publisher.programIngeniería en Energíaspa
dc.publisher.programIngeniería de Telecomunicacionesspa
dc.publisher.programIngeniería Ambientalspa
dc.relation.citationissue1
dc.relation.citationvolume18
dc.relation.isversionofhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-105012864876&doi=10.1007%2fs12155-025-10873-5&partnerID=40&md5=b7392be0245fd75a471a8aa84d2c481a
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dc.rights.accesoRestricted access
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceBioenergy Research
dc.sourceBioenergy Res.
dc.sourceScopus
dc.subjectCattle manure
dc.subjectEnzymatic catalysis
dc.subjectKinetic model
dc.subjectMass transfer phenomena
dc.subjectBiomolecules
dc.subjectBiopolymers
dc.subjectBoundary layers
dc.subjectCatalysis
dc.subjectDiffusion in liquids
dc.subjectFertilizers
dc.subjectHydrolysis
dc.subjectKinetic parameters
dc.subjectKinetic theory
dc.subjectAnaerobic digestion process
dc.subjectAnaerobics
dc.subjectCattle manures
dc.subjectEnzymatic catalysis
dc.subjectFirst-order models
dc.subjectKinetic models
dc.subjectLivestock manure
dc.subjectMass transfer phenomena
dc.subjectOptimal operating conditions
dc.subjectTraditional approachs
dc.subjectAnaerobic digestion
dc.subjectManures
dc.titleTheoretical Analysis of the Hydrolysis Mechanism of Biopolymers in the Anaerobic Digestion Process of Livestock Manure
dc.typeArticle
dc.type.localArtículospa
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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